CCDC88C Polyclonal antibody

CCDC88C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25769-1-AP

Synonyms

CCDC88C, DAPLE, hDaple, HkRP2, Hook related protein 2, KIAA1509, Protein Daple

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Validation Data Gallery

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  WB analysis of HEK-293 using 25769-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25769-1-AP (CCDC88C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 25769-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25769-1-AP (CCDC88C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 25769-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25769-1-AP (CCDC88C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 25769-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 25769-1-AP (CCDC88C antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 25769-1-AP is CCDC88C Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC88C fusion protein Ag22711
• Full Name: coiled-coil domain containing 88C
• Calculated Molecular Weight: 2028 aa, 228 kDa
• Observed Molecular Weight: 250 kDa, 100 kDa
• GenBank Accession Number: BC028565
• Gene Symbol: CCDC88C
• Gene ID (NCBI): 440193
• RRID: AB_2880233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Nat Commun; EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia.; Authors - Katie S Kindt
mouse		Sci Adv; RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells; Authors - Anil Akturk