CCDC9 Polyclonal antibody

• 

  WB analysis of HeLa using 21104-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21104-1-AP (CCDC9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21104-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21104-1-AP (CCDC9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 21104-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21104-1-AP (CCDC9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 21104-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21104-1-AP (CCDC9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

CCDC9 Polyclonal Antibody for WB, ELISA

Cat No. 21104-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CCDC9

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human skeletal muscle tissue, mouse small intestine tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21104-1-AP targets CCDC9 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15374

  Product name: Recombinant human CCDC9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 185-531 aa of BC002787

  Sequence: VLEPNPVRNFLDDPRRRSGPLEESERDRREESRRHGRNWGGPDFERVRCGLEHERQGRRAGLGSAGDMTLSMTGRERSEYLRWKQEREKIDQERLQRHRKPTGQWRREWDAEKTDGMFKDGPVPAHEPSHRYDDQAWARPPKPPTFGEFLSQHKAEASSRRRRKSSRPQAKAAPRAYSDHDDRWETKEGAASPAPETPQPTSPETSPKETPMQPPEIPAPAHRPPEDEGEENEGEEDEEWEDISEDEEEEEIEVEEGDEEEPAQDHQAPEAAPTGIPCSEQAHGVPFSPEEPLLEPQAPGTPSSPFSPPSGHQPVSDWGEEVELNSPRTTHLAGALSPGEAWPFESV

• Full Name: coiled-coil domain containing 9
• Calculated Molecular Weight: 531 aa, 60 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC002787
• Gene Symbol: CCDC9
• Gene ID (NCBI): 26093
• RRID: AB_10858920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y3X0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC9 is Coiled-coil domain-containing protein 9 which functions on RNA binding. The MW of CCDC9 is about 60-70 kDa in Western blot detection.