Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, human testis tissue, mouse colon tissue, Jurkat cells, human testis tissues |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human colon tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20861-1-AP targets CCDC93 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14855 Product name: Recombinant human CCDC93 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 282-631 aa of BC028609 Sequence: CSAEIKQIVSEYAEKQSELSAEESPEKLGTSQLHRRKVISLNKQIAQKTKHLEELRASHTSLQARYNEAKKTLTELKTYSEKLDKEQAALEKIESKADPSILQNLRALVAMNENLKSQEQEFKAHCREEMTRLQQEIENLKAERAPRGDEKTLSSGEPPGTLTSAMTHDEDLDRRYNMEKEKLYKIRLLQARRNREIAILHRKIDEVPSRAELIQYQKRFIELYRQISAVHKETKQFFTLYNTLDDKKVYLEKEISLLNSIHENFSQAMASPAARDQFLRQMEQIVEGIKQSRMKMEKKKQENKMRRDQLNDQYLELLEKQRLYFKTVKEFKEEGRKNEMLLSKVKAKAS Predict reactive species |
| Full Name | coiled-coil domain containing 93 |
| Calculated Molecular Weight | 631 aa, 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC028609 |
| Gene Symbol | CCDC93 |
| Gene ID (NCBI) | 54520 |
| RRID | AB_10696446 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q567U6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCDC93 antibody 20861-1-AP | Download protocol |
| IP protocol for CCDC93 antibody 20861-1-AP | Download protocol |
| WB protocol for CCDC93 antibody 20861-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-COMMD1 antibody (Cat# 20861-1-AP) has 20 citations across journals including Cell, Nature Cell Biology, Nature Communications, Science Immunology, eLife, Journal of Cell Biology, and Molecular Biology of the Cell. Research fields span endosomal trafficking and recycling, Commander complex structure, immune regulation, copper homeostasis, viral entry, Notch signaling, ovarian cancer, insulin secretion, phagolysosomal maturation, and mitochondrial dysfunction. Applications are predominantly WB, with some CoIP, IF, and IHC.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling. | ||
J Extracell Vesicles Genome-Wide CRISPR/Cas9 Screening Identifies the COMMANDER Recycling Complex as a Key Player in EV Uptake.
| ||
Nat Commun CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL. | ||
Sci Immunol Celastrol suppresses humoral immune responses and autoimmunity by targeting the COMMD3/8 complex | ||
Mol Metab Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion. |
