CCDC94 Monoclonal antibody, PBS Only

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  WB analysis using 68324-1-Ig (same clone as 68324-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68324-1-Ig (CCDC94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68324-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68324-1-Ig (same clone as 68324-1-PBS)

  WB result of CCDC94 antibody (68324-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC94 transfected HeLa cells. This data was developed using the same antibody clone with 68324-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68324-1-Ig (same clone as 68324-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC94 antibody (68324-1-Ig, Clone: 1D1G9 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 68324-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CCDC94 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68324-1-PBS

Clone No.1D1G9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

YJU2, Coiled-coil domain-containing protein 94, 1D1G9

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Product Information

68324-1-PBS targets CCDC94 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13772

  Product name: Recombinant human CCDC94 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-323 aa of BC000561

  Sequence: MSERKVLNKYYPPDFDPSKIPKLKLPKDRQYVVRLMAPFNMRCKTCGEYIYKGKKFNARKETVQNEVYLGLPIFRFYIKCTRCLAEITFKTDPENTDYTMEHGATRNFQAEKLLEEEEKRVQKEREDEELNNPMKVLENRTKDSKLEMEVLENLQELKDLNQRQAHVDFEAMLRQHRLSEEERRRQQQEEDEQETAALLEEARKRRLLEDSDSEDEAAPSPLQPALRPNPTAILDEAPKPKRKVEVWEQSVGSLGSRPPLSRLVVVKKAKADPDCSNGQPQAAPTPGAPQNRKEANPTPLTPGASSLSQLGAYLDSDDSNGSN

• Full Name: coiled-coil domain containing 94
• Calculated Molecular Weight: 323 aa, 37 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC000561
• Gene Symbol: CCDC94
• Gene ID (NCBI): 55702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BW85
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)