CCHCR1 Polyclonal antibody

CCHCR1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21869-1-AP

Synonyms

Putative gene 8 protein, Pg8, HCR, Coiled-coil alpha-helical rod protein 1, C6orf18

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Validation Data Gallery

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  IHC staining of human colon using 21869-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21869-1-AP (CCHCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 21869-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21869-1-AP (CCHCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 21869-1-AP

  Immunofluorescent analysis of HEK-293 cells using 21869-1-AP (CCHCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21869-1-AP targets CCHCR1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCHCR1 fusion protein Ag16141
• Full Name: coiled-coil alpha-helical rod protein 1
• Calculated Molecular Weight: 871 aa, 99 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC110534
• Gene Symbol: CCHCR1
• Gene ID (NCBI): 54535
• RRID: AB_2878932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCHCR1, also named as C6orf18 or Putative gene 8 protein, is a 782 amino acid protein, which is a nuclear protein abundantly expressed in heart, liver, skeletal muscle, kidney and pancreas, and to a lesser extent in lung and placenta. HCR is overexpressed in keratinocytes of psoriatic lesions. HCR is associated with susceptibility to psoriasis, a chronic inflammatory dermatosis that affects approximately 2% of the population. CCHCR1 may be a regulator of keratinocyte proliferation or differentiation.