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Calicin Polyclonal antibody

Calicin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

calicin, CCIN

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

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Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inhuman testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13972-1-AP targets Calicin in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5005

Product name: Recombinant human CCIN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC019251

Sequence: MKLEFTEKNYNSFVLQNLNRQRKRKEYWDMALSVDNHVFFAHRNVLAAVSPLVRSLISSNDMKTADELFITIDTSYLSPVTVDQLLDYFYSGKVVISEQNVEELLRGAQYFNTPRLRVHCNDFLIKSICRANCLRYLFLAELFELKEVSDVAYSGIRDNFHYWASPEGSMHFMRCPPVIFGRLLRDENLHVLNEDQALSALINWVYFRKEDREKYFKKFFNYINLNAVSNKTLVFASNKLVGMENTSSHTTLIESVLMDRKQERPCSLLVYQRKGALLDSVVILGGQKAHGQFNDGVFAYIIQENLWMELSDMPYRAAALSATSAGRYIYISGGTTEQISGLKTAWRYDMD

Predict reactive species
Full Name calicin
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC019251
Gene Symbol Calicin
Gene ID (NCBI) 881
RRIDAB_10696308
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13939
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Calicin antibody 13972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The Calicin Polyclonal antibody (Cat# 13972-1-AP) has 3 total citations. These appear in Ecotoxicology and Environmental Safety, Zoological Research, and Clinical Genetics. The research fields span reproductive biology and spermatogenesis (sperm head shaping, perinuclear theca protein function), ecotoxicology (PFOA exposure effects on sperm), and clinical genetics (homozygous CCIN variant causing male infertility with abnormal sperm head morphology).

SpeciesApplicationTitle
mouseWB

Ecotoxicol Environ Saf

PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice.

Authors - Chuan Wang
mouseWB,IF

Zool Res

Perinuclear theca protein FNDC8 interacts with CCIN and ACTL7A to ensure proper sperm head shaping during spermiogenesis.

Authors - Jia-Yi Liu
humanWB,IF

Clin Genet

Novel homozygous variant of CCIN causes male infertility owing to the abnormal sperm head with a nuclear subsidence phenotype

Authors - Jiaxin He
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