Tested Applications
| Positive WB detected in | human testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13972-1-AP targets Calicin in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5005 Product name: Recombinant human CCIN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC019251 Sequence: MKLEFTEKNYNSFVLQNLNRQRKRKEYWDMALSVDNHVFFAHRNVLAAVSPLVRSLISSNDMKTADELFITIDTSYLSPVTVDQLLDYFYSGKVVISEQNVEELLRGAQYFNTPRLRVHCNDFLIKSICRANCLRYLFLAELFELKEVSDVAYSGIRDNFHYWASPEGSMHFMRCPPVIFGRLLRDENLHVLNEDQALSALINWVYFRKEDREKYFKKFFNYINLNAVSNKTLVFASNKLVGMENTSSHTTLIESVLMDRKQERPCSLLVYQRKGALLDSVVILGGQKAHGQFNDGVFAYIIQENLWMELSDMPYRAAALSATSAGRYIYISGGTTEQISGLKTAWRYDMD Predict reactive species |
| Full Name | calicin |
| Calculated Molecular Weight | 67 kDa |
| Observed Molecular Weight | 60-65 kDa |
| GenBank Accession Number | BC019251 |
| Gene Symbol | Calicin |
| Gene ID (NCBI) | 881 |
| RRID | AB_10696308 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13939 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Calicin antibody 13972-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Calicin Polyclonal antibody (Cat# 13972-1-AP) has 3 total citations. These appear in Ecotoxicology and Environmental Safety, Zoological Research, and Clinical Genetics. The research fields span reproductive biology and spermatogenesis (sperm head shaping, perinuclear theca protein function), ecotoxicology (PFOA exposure effects on sperm), and clinical genetics (homozygous CCIN variant causing male infertility with abnormal sperm head morphology).
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice. | ||
Zool Res Perinuclear theca protein FNDC8 interacts with CCIN and ACTL7A to ensure proper sperm head shaping during spermiogenesis. | ||
Clin Genet Novel homozygous variant of CCIN causes male infertility owing to the abnormal sperm head with a nuclear subsidence phenotype |





