Calicin Polyclonal antibody

• 

  WB analysis of human testis using 13972-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13972-1-AP (Calicin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 13972-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 13972-1-AP (Calicin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 13972-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13972-1-AP (Calicin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Calicin Polyclonal Antibody for WB, ELISA

Cat No. 13972-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

calicin, CCIN

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Tested Applications

• Positive WB detected in: human testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

13972-1-AP targets Calicin in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5005

  Product name: Recombinant human CCIN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC019251

  Sequence: MKLEFTEKNYNSFVLQNLNRQRKRKEYWDMALSVDNHVFFAHRNVLAAVSPLVRSLISSNDMKTADELFITIDTSYLSPVTVDQLLDYFYSGKVVISEQNVEELLRGAQYFNTPRLRVHCNDFLIKSICRANCLRYLFLAELFELKEVSDVAYSGIRDNFHYWASPEGSMHFMRCPPVIFGRLLRDENLHVLNEDQALSALINWVYFRKEDREKYFKKFFNYINLNAVSNKTLVFASNKLVGMENTSSHTTLIESVLMDRKQERPCSLLVYQRKGALLDSVVILGGQKAHGQFNDGVFAYIIQENLWMELSDMPYRAAALSATSAGRYIYISGGTTEQISGLKTAWRYDMD

• Full Name: calicin
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC019251
• Gene Symbol: Calicin
• Gene ID (NCBI): 881
• RRID: AB_10696308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13939
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Clin Genet; Novel homozygous variant of CCIN causes male infertility owing to the abnormal sperm head with a nuclear subsidence phenotype; Authors - Jiaxin He