CCKAR-specific Polyclonal antibody

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  IHC staining of mouse pancreas using 16550-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 16550-1-AP (CCKAR-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 16550-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16550-1-AP (CCKAR-specific Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 16550-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16550-1-AP (CCKAR-specific Antibody) at dilution of 1:50 (under 40x lens).

CCKAR-specific Polyclonal Antibody for IHC, ELISA

Cat No. 16550-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

CCKAR, CCK A, CCK A receptor, CCK AR, CCK1 R

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Tested Applications

• Positive IHC detected in: human stomach tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16550-1-AP targets CCKAR-specific in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cholecystokinin A receptor
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: NM_000730
• Gene Symbol: CCKAR
• Gene ID (NCBI): 886
• RRID: AB_2878275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P32238
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cholecystokinin (CCK) receptor, including CCKAR and CCKBR, is a receptor for cholecystokinin, mainly regulating pancreatic growth and enzyme secretion, and smooth muscle contraction of the gallbladder and stomach (PMID: 34556863). CCKAR (Cholecystokinin receptor type A) is primarily expressed in the alimentary tract, while CCKBR is mainly found in the brain and the stomach (PMID: 16816139). CCKAR is a biomarker for prognosis and asynchronous brain metastasis of non-small cell lung cancer (PMID: 36733361).