CCKBR-specific Polyclonal antibody

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• CCKBR-specific Polyclonal antibody (16549-1-AP)

CCKBR-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16549-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CCKBR, CCK B, CCK B receptor, CCK BR, CCK2 R

• 

  WB analysis of BxPC-3 using 16549-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SGC-7901 using 16549-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human stomach using 16549-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 16549-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16549-1-AP (CCKBR-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 16549-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CCKBR-specific antibody (16549-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of BxPC-3 using 16549-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 16549-1-AP (CCKBR-specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: BxPC-3 cells, human stomach tissue, SGC-7901 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16549-1-AP targets CCKBR-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cholecystokinin B receptor
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 64 kDa, 50 kDa
• GenBank Accession Number: NM_176875
• Gene Symbol: CCKBR
• Gene ID (NCBI): 887
• RRID: AB_2070508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P32239
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cholecystokinin B receptor (CCKBR) is a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors.

Publications

What published studies show

The anti-CCK2R antibody (Cat# 16549-1-AP) has 3 total citations. These publications appear in Cell (IF 45.1), Molecular Pharmaceutics (IF 4.9), and Journal of Cancer Research and Clinical Oncology (IF 3.3). The associated research fields include Alzheimer's disease treatment via biased CCKBR agonism, and colorectal cancer diagnostics and immunotherapy targeting CCK2R-expressing malignancies. Applications span WB, IHC, and IF in both mouse and human samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IHC,IF	Cell; Elucidating pathway-selective biased CCKBR agonism for Alzheimer's disease treatment.; Authors - Jia-Le Wang
human	WB,IHC	Mol Pharm; Pseudomonas Exotoxin A-Based Immunotherapy Targeting CCK2R-Expressing Colorectal Malignancies: An In Vitro and In Vivo Evaluation.; Authors - Jiang Chang
human	IF	J Cancer Res Clin Oncol; Cholecystokinin type 2 receptor in colorectal cancer: diagnostic and therapeutic target.; Authors - Jiang Chang