CCKBR-specific Polyclonal antibody

CCKBR-specific Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16549-1-AP

Synonyms

CCK B, CCK B receptor, CCK BR, CCK2 R, CCK2R, CCKBR, CCKRB, Cholecystokinin 2 receptor, cholecystokinin B receptor, GASR



Tested Applications

Positive WB detected inBxPC-3 cells, human stomach tissue, SGC-7901 cells
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16549-1-AP targets CCKBR-specific in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cholecystokinin B receptor
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 64 kDa, 50 kDa
GenBank Accession NumberNM_176875
Gene Symbol CCKBR
Gene ID (NCBI) 887
RRIDAB_2070508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cholecystokinin B receptor (CCKBR) is a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors.

Protocols

Product Specific Protocols
WB protocol for CCKBR-specific antibody 16549-1-APDownload protocol
IF protocol for CCKBR-specific antibody 16549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Mol Pharm

Pseudomonas Exotoxin A-Based Immunotherapy Targeting CCK2R-Expressing Colorectal Malignancies: An In Vitro and In Vivo Evaluation.

Authors - Jiang Chang
humanIF

J Cancer Res Clin Oncol

Cholecystokinin type 2 receptor in colorectal cancer: diagnostic and therapeutic target.

Authors - Jiang Chang