Filter: AllIHCIF/ICC

IHC staining of human testis using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin cancer using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin cancer using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 22301-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A431 using 22301-1-AP
Immunofluorescent analysis of A431 cells using 22301-1-AP (CCL27 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of A431 using 22301-1-AP
Immunofluorescent analysis of A431 cells using 22301-1-AP (CCL27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CCL27 Polyclonal antibody

Name: CCL27 Polyclonal antibody
Brand: Proteintech
SKU: 22301-1-AP

CCL27 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 22301-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

ALP, C C motif chemokine 27, CC chemokine ILC, CCL27, CTACK, CTAK, ESKINE, IL 11 R alpha locus chemokine, ILC, PESKY, SCYA27, Skinkine, Small inducible cytokine A27

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in	human testis tissue, human liver tissue, human liver cancer tissue, human skin tissue, human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22301-1-AP targets CCL27 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	chemokine (C-C motif) ligand 27
Calculated Molecular Weight	13 kDa
GenBank Accession Number	NM_006664
Gene Symbol	CCL27
Gene ID (NCBI)	10850
RRID	AB_2879065
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y4X3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCL27, also known as CTACK, is a member of the CC chemokine subfamily. CCL27 is predominantly expressed in skin and selectively attracts cutaneous lymphocyte antigen-positive T cells and Langerhans cells. The CCL27 secreted from keratinocytes was reportedly involved in inflammatory skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. CCL27 is a ligand to CCR10. The binding of CCL27 to CCR10 on T cells promotes their migration to the skin, where they can then participate in local immune responses.