RANTES Polyclonal antibody

RANTES Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, Neutralization, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12000-1-AP

Synonyms

C C motif chemokine 5, CCL5, chemokine (C C motif) ligand 5, D17S136E, EoCP, RANTES, SCYA5, SIS delta, SISd, Small inducible cytokine A5, T cell specific protein P228, T cell specific protein RANTES, TCP228

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Validation Data Gallery

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  WB analysis of human peripheral blood platelets using 12000-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12000-1-AP (RANTES antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human peripheral blood platelets

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

12000-1-AP targets RANTES in WB, Neutralization, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RANTES fusion protein Ag2617
• Full Name: chemokine (C-C motif) ligand 5
• Calculated molecular weight: 91 aa, 10 kDa
• Observed molecular weight: 8 kDa
• GenBank accession number: BC008600
• Gene symbol: CCL5
• Gene ID (NCBI): 6352
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Adv Healthc Mater; A Novel Tri-functional Liposome Re-educates "Cold Tumor" and Abrogates Tumor Growth by Synergizing Autophagy Inhibition and PD-L1 Blockade; Authors - Fei Zhou
mouse	WB,IF	Stem Cells; Targeted deletion of Rictor in BMSCs reduces the biological activity of K7M2 cells and mitigates OS-induced bone destruction; Authors - Jinhong Lu
human	Neutralization	Ann Transl Med; Upregulation of STAT1-CCL5 axis is a biomarker of colon cancer and promotes the proliferation of colon cancer cells.; Authors - Mengke Niu
human	Neutralization	Oncol Rep; Effects of CCL5 on the biological behavior of breast cancer and the mechanisms of its interaction with tumor‑associated macrophages.; Authors - Gaili An
human,mouse	IHC	Research (Wash D C); PTBP2-Mediated Alternative Splicing of IRF9 Controls Tumor-Associated Monocyte/Macrophage Chemotaxis and Repolarization in Neuroblastoma Progression; Authors - Jue Tang
human	IHC	J Immunother Cancer; Chordoma recruits and polarizes tumor-associated macrophages via secreting CCL5 to promote malignant progression; Authors - Jiuhui Xu