RANTES Polyclonal antibody

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  WB analysis using 12000-1-AP

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12000-1-AP (RANTES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RANTES Polyclonal Antibody for WB, ELISA

Cat No. 12000-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, Neutralization, ELISA

Synonyms

C C motif chemokine 5, CCL5, chemokine (C C motif) ligand 5, D17S136E, EoCP, RANTES, SCYA5, SIS delta, SISd, Small inducible cytokine A5, T cell specific protein P228, T cell specific protein RANTES, TCP228

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Tested Applications

• Positive WB detected in: human peripheral blood platelets

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Product Information

12000-1-AP targets RANTES in WB, IHC, IF, Neutralization, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RANTES fusion protein Ag2617
• Full Name: chemokine (C-C motif) ligand 5
• Calculated Molecular Weight: 91 aa, 10 kDa
• Observed Molecular Weight: 8 kDa
• GenBank Accession Number: BC008600
• Gene Symbol: CCL5/RANTES
• Gene ID (NCBI): 6352
• RRID: AB_2877815
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13501
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RANTES (CCL5) belongs to the CC chemokine family and induces leukocyte migration by binding to specific receptors in the G protein-coupled receptor family. RANTES (CCL5) has been shown in vitro to mediate eosinophil, lymphocyte, neutrophil, and monocyte chemotaxis, and it initiates several other proinflammatory events, such as integrin activation, lipid mediator biosynthesis, and degranulation.

Publications

Species	Application	Title
mouse	WB	Brain Behav Immun; Egln3 expression in microglia enhances the neuroinflammatory responses in Alzheimer's disease; Authors - Jiaxin Guan
rat	WB	Phytomedicine; Integration of transcriptomics and metabolomics reveals the mechanism of Glycyrrhizae Radix Et Rhizoma extract inhibiting CCL5 in the treatment of acute pharyngitis; Authors - Xiaoxiao Han
mouse	IHC	Immunology; NME4 suppresses NFκB2-CCL5 axis, restricting CD8+ T cell tumour infiltration in oesophageal squamous cell carcinoma; Authors - Shutao Zheng
		iScience; Cancer-associated fibroblasts promote enzalutamide resistance and PD-L1 expression in prostate cancer through CCL5-CCR5 paracrine axis; Authors - Zhi Xiong
mouse	WB,IF	Stem Cells; Targeted deletion of Rictor in BMSCs reduces the biological activity of K7M2 cells and mitigates OS-induced bone destruction; Authors - Jinhong Lu
human	Neutralization	Ann Transl Med; Upregulation of STAT1-CCL5 axis is a biomarker of colon cancer and promotes the proliferation of colon cancer cells.; Authors - Mengke Niu