CCM2 Polyclonal antibody

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  WB analysis of MCF-7 using 26270-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26270-1-AP (CCM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26270-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26270-1-AP (CCM2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26270-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26270-1-AP (CCM2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 26270-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 26270-1-AP (CCM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

CCM2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 26270-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C7orf22, CCM2, Malcavernin, OSM, PP10187

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below

Product Information

26270-1-AP targets CCM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24233

  Product name: Recombinant human CCM2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 88-298 aa of BC004903

  Sequence: ILHFIDNAKRAHQLPGHLTQEHDAVLSLSAYNVKLAWRDGEDIILRVPIHDIAAVSYVRDDAAHLVVLKTAQDPGISPSQSLCAESSRGLSAGSLSESAVGPVEACCLVILAAESKVAAEELCCLLGQVFQVVYTESTIDFLDRAIFDGASTPTHHLSLHSDDSSTKVDIKETYEVEASTFCFPESVDVGGASPHSKTISESELSASATEL

• Full Name: cerebral cavernous malformation 2
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC004903
• Gene Symbol: CCM2
• Gene ID (NCBI): 83605
• RRID: AB_2880454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BSQ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Endocrinology; Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice; Authors - Abraham Behrmann ; KD Validated
human	WB	Biochem J; CCM2 and PAK4 act downstream of atrial natriuretic peptide signaling to promote cell spreading.; Authors - Koichi Miura ; KD Validated