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Cyclin A2 Polyclonal antibody

Cyclin A2 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (2)





Cat no : 18202-1-AP


CCN1, CCNA, CCNA2, Cyclin A, cyclin A2, cyclinA2

Tested Applications

Positive WB detected inHL-60 cells, NIH/3T3 cells, HeLa cells, K-562 cells, U-937 cells, MCF-7 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

18202-1-AP targets Cyclin A2 in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin A2 fusion protein Ag12765
Full Name cyclin A2
Calculated molecular weight 432 aa, 49 kDa
Observed molecular weight 48-52 kDa
GenBank accession numberBC104783
Gene symbol CCNA2
Gene ID (NCBI) 890
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.


Product Specific Protocols
WB protocol for Cyclin A2 antibody 18202-1-APDownload protocol
IHC protocol for Cyclin A2 antibody 18202-1-APDownload protocol
IF protocol for Cyclin A2 antibody 18202-1-APDownload protocol
IP protocol for Cyclin A2 antibody 18202-1-APDownload protocol
FC protocol for Cyclin A2 antibody 18202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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