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Cyclin A2 Antibody

Cyclin A2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

IF, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 18202-1-AP

Synonyms

CCN1, CCNA, CCNA2, Cyclin A, cyclin A2



Tested Applications

Positive WB detected inHL-60 cells, NIH/3T3 cells, HeLa cells, K-562 cells, U-937 cells, MCF-7 cells
Positive IP detected inHL-60 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18202-1-AP targets Cyclin A2 in IF, IP, WB, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin A2 fusion protein Ag12765
Full Name cyclin A2
Calculated molecular weight 432aa,49 kDa
Observed molecular weight 48-52 kDa
GenBank accession number BC104783
Gene symbol CCNA2
Gene ID (NCBI) 890
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.

Protocols

Product Specific Protocols
WB protocol for Cyclin A2 antibody 18202-1-APDownload protocol
IF protocol for Cyclin A2 antibody 18202-1-APDownload protocol
IP protocol for Cyclin A2 antibody 18202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Stem Cell Res Ther

Fibroblast growth factor-5 promotes spermatogonial stem cell proliferation via ERK and AKT activation.

Authors - Ruhui Tian
humanWB

Cancers (Basel)

Metformin Treatment Suppresses Melanoma Cell Growth and Motility Through Modulation of microRNA Expression.

Authors - Hui-Wen Tseng
humanWB

Biomed Pharmacother

Lin28 promotes dental pulp cell proliferation via upregulation of cyclin-dependent proteins and interaction with let-7a/IGF2BP2 pathways.

Authors - Yan Liu
humanWB

Int J Biol Sci

RRM2 is a potential prognostic biomarker with functional significance in glioma.

Authors - Hongzhi Sun
humanWB

Sci Rep

Low C6orf141 Expression is Significantly Associated with a Poor Prognosis in Patients with Oral Cancer.

Authors - Cheng-Mei Yang
mouseWB

Cell Death Discov

Celastrol attenuates ox-LDL-induced mesangial cell proliferation via suppressing NLRP3 inflammasome activation.

Authors - Zhenzhen Sun