Validation Data Gallery
|Positive WB detected in||Caco-2 cells, MCF-7 cells, NIH/3T3 cells, U2OS cells, HEK-293 cells|
|Positive IHC detected in||human renal cell carcinoma tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
10934-1-AP targets Cyclin D2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, bovine, goat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cyclin D2 fusion protein Ag1377|
|Full Name||cyclin D2|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC010958|
|Gene ID (NCBI)||894|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.
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