Validation Data Gallery
|Positive WB detected in||Caco-2 cells, MCF-7 cells, NIH/3T3 cells, U2OS cells, HEK-293 cells|
|Positive IHC detected in||human renal cell carcinoma tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
10934-1-AP targets Cyclin D2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, bovine, goat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cyclin D2 fusion protein Ag1377|
|Full Name||cyclin D2|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC010958|
|Gene ID (NCBI)||894|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.
SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma.
The Oncogenic Transcription Factor RUNX1/ETO Corrupts Cell Cycle Regulation to Drive Leukemic Transformation.
LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.
Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.
Transcription factor MafB may play an important role in secondary hyperparathyroidism.
Int J Cancer
Human tumor microRNA signatures derived from large-scale oligonucleotide microarray datasets.