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  • KD/KO Validated

Cyclin D2 Polyclonal antibody

Cyclin D2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10934-1-AP

Synonyms

CCND2, cyclin D, cyclin D2, G1/S-specific cyclin-D2



Tested Applications

Positive WB detected inCaco-2 cells, MCF-7 cells, NIH/3T3 cells, U2OS cells, HEK-293 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

10934-1-AP targets Cyclin D2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, bovine, goat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin D2 fusion protein Ag1377
Full Name cyclin D2
Calculated molecular weight 32 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC010958
Gene symbol CCND2
Gene ID (NCBI) 894
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.

Protocols

Product Specific Protocols
WB protocol for Cyclin D2 antibody 10934-1-APDownload protocol
IHC protocol for Cyclin D2 antibody 10934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma.

Authors - Soufiane Boumahdi
humanWB

Cancer Cell

The Oncogenic Transcription Factor RUNX1/ETO Corrupts Cell Cycle Regulation to Drive Leukemic Transformation.

Authors - Natalia Martinez-Soria
  • KD Validated
humanWB

Mol Cancer

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

Authors - Xi Jin
mouseWB

Dev Cell

Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.

Authors - Yan Gong
mouseIHC

Kidney Int

Transcription factor MafB may play an important role in secondary hyperparathyroidism.

Authors - Naoki Morito
humanWB

Int J Cancer

Human tumor microRNA signatures derived from large-scale oligonucleotide microarray datasets.

Authors - Wang Wenzhang W