Cyclin G Polyclonal antibody

Cyclin G Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10897-1-AP

Synonyms

CCNG, CCNG1, CYCG1, Cyclin G, cyclin G1

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Validation Data Gallery

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  WB analysis of MDA-MB-453s using 10897-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 10897-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10897-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10897-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10897-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10897-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10897-1-AP (Cyclin G antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10897-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10897-1-AP (Cyclin G antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, mouse ovary tissue, HeLa cells, mouse liver tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

10897-1-AP targets Cyclin G in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin G fusion protein Ag1157
• Full Name: cyclin G1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC007093
• Gene Symbol: CCNG1
• Gene ID (NCBI): 900
• RRID: AB_2228613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; miR-488-3p Protects Cardiomyocytes against Doxorubicin-Induced Cardiotoxicity by Inhibiting CyclinG1.; Authors - Mingjing Yan
human	WB	Cancer Lett; The C/EBPβ-LINC01133 axis promotes cell proliferation in pancreatic ductal adenocarcinoma through upregulation of CCNG1.; Authors - Chen-Song Huang
human	WB	J Biol Chem; MicroRNA-26b Inhibits Hepatitis B Virus Transcription and Replication by Targeting the Host Factor CHORDC1.; Authors - Fanpeng Zhao
human	WB	Exp Cell Res; PRMT6 functionally associates with PRMT5 to promote colorectal cancer progression through epigenetically repressing the expression of CDKN2B and CCNG1; Authors - Yuzhong Chen
human	WB	Oncol Res; Calcyclin-binding protein contributes to cholangiocarcinoma progression by inhibiting ubiquitination of MCM2; Authors - Yusen Zhang