CCNL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CCNL2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11212-1-AP

Synonyms

ANIA 6B, CCNL2, CCNM, cyclin L2, HCLA ISO, HLA ISO, PCEE, SB138

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Validation Data Gallery

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  WB analysis using 11212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11212-1-AP (CCNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 11212-1-AP

  WB result of CCNL2 antibody (11212-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCNL2 transfected A375 cells.

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Tested Applications

• Positive WB detected in: A375 cells, HeLa cells, U-251 cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11212-1-AP targets CCNL2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCNL2 fusion protein Ag1710
• Full Name: cyclin L2
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC016333
• Gene Symbol: CCNL2
• Gene ID (NCBI): 81669
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNL2 (Cyclin-L2) is also named as SB138 and Paneth cell-enhanced expression protein. Human cyclin L2 is a novel member of the cyclin family. Human cyclin L2 shares significant homology to cyclin L1, K, T1, T2, and C, which are involved in transcriptional regulation via phosphorylation of the C-terminal domain of RNA polymerase II (PMID: 14684736). Cyclin L2 co-localizes with splicing factors SC-35 and 9G8 within nuclear speckles and that it associates with hyperphosphorylated, but not hypophosphorylated, RNA polymerase II and CDK p110 PITSLRE kinase via its N-terminal cyclin domains (PMID: 14684736). Human cyclin L2 is expressed ubiquitously in normal human tissues and tumor cells.