CCR2a-specific Recombinant antibody

• 

  WB analysis using 84911-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84911-3-RR (CCR2a-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CCR2a-specific {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84911-3-RR

Clone No.242217G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CCR2, C-C CKR-2, CC CKR 2, C-C chemokine receptor type 2, C C CKR 2

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Tested Applications

• Positive WB detected in: U-937 cells, THP-1 cells, A431 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84911-3-RR targets CCR2a-specific in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: chemokine (C-C motif) receptor 2
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_000647
• Gene Symbol: CCR2
• Gene ID (NCBI): 729230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P41597
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates.