CCR7 Polyclonal antibody

CCR7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25898-1-AP

Synonyms

BLR2, C C chemokine receptor type 7, C C CKR 7, CC CKR 7, CCR 7, CCR7, CD197, CDw197, CMKBR7, EBI1, EVI1, MIP 3 beta receptor



Tested Applications

Positive WB detected inMCF-7 cells, K-562 cells, C6 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25898-1-AP targets CCR7 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCR7 fusion protein Ag22941
Full Name chemokine (C-C motif) receptor 7
Calculated Molecular Weight 378 aa, 43 kDa
Observed Molecular Weight 40-43 kDa
GenBank Accession NumberBC035343
Gene Symbol CCR7
Gene ID (NCBI) 1236
RRIDAB_2880289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-C chemokine receptor type 7 (CCR7), which belongs to the G-protein coupled receptor 1 family, is also a receptor for the MIP-3-beta chemokine, and probably acts as a mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. CCR7 is specially expressed in various lymphoid tissues and activated B- and T-lymphocytes, and can be strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

Protocols

Product Specific Protocols
WB protocol for CCR7 antibody 25898-1-APDownload protocol
IHC protocol for CCR7 antibody 25898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Bioact Mater

Two-stage degradation and novel functional endothelium characteristics of a 3-D printed bioresorbable scaffold.

Authors - Tieying Yin
mouseIHC

Virulence

Intranasal bovine β-defensin-5 enhances antituberculosis immunity in a mouse model by a novel protein-based respiratory mucosal vaccine.

Authors - Zhengmin Liang
ratIHC

Front Cell Dev Biol

Potential Mechanisms of the Impact of Hepatocyte Growth Factor Gene-Modified Tendon Stem Cells on Tendon Healing.

Authors - Mingzhao Zhang
ratIF

Stem Cell Res Ther

Tendon stem cell-derived exosomes regulate inflammation and promote the high-quality healing of injured tendon.

Authors - Mingzhao Zhang
humanIHC

Front Mol Biosci

CCR7 Has Potential to Be a Prognosis Marker for Cervical Squamous Cell Carcinoma and an Index for Tumor Microenvironment Change.

Authors - Wei-Jie Tian
humanWB

Front Pharmacol

MicroRNA-532-3p Suppresses Malignant Behaviors of Tongue Squamous Cell Carcinoma via Regulating CCR7.

Authors - Cuijuan Feng