CCS Polyclonal antibody

CCS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22802-1-AP

Synonyms

CCS, SOD 4, SOD4, Superoxide Dismutase 4



Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse brain tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

22802-1-AP targets CCS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCS fusion protein Ag18850
Full Name copper chaperone for superoxide dismutase
Calculated Molecular Weight 274 aa, 29 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC105016
Gene Symbol CCS
Gene ID (NCBI) 9973
RRIDAB_2879172
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.

Protocols

Product Specific Protocols
WB protocol for CCS antibody 22802-1-APDownload protocol
IHC protocol for CCS antibody 22802-1-APDownload protocol
IF protocol for CCS antibody 22802-1-APDownload protocol
IP protocol for CCS antibody 22802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Stem Cell

Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.

Authors - Rashim Pal Singh
humanWB

Neurobiol Dis

The M1311V variant of ATP7A is associated with impaired trafficking and copper homeostasis in models of motor neuron disease.

Authors - Nadine Bakkar
mouseWB

Sci Rep

S-acylation of SOD1, CCS, and a stable SOD1-CCS heterodimer in human spinal cords from ALS and non-ALS subjects.

Authors - Sarah E Antinone
humanWB

Br J Cancer

AMPK phosphorylates and stabilises copper transporter 1 to synergise metformin and copper chelator for breast cancer therapy

Authors - Xiaomei Zhang
mouseWB

Mol Med Rep

Artemether ameliorates adriamycin induced cardiac atrophy in mice

Authors - Wenci Weng