CCT3 Recombinant monoclonal antibody

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• CCT3 Recombinant monoclonal antibody (87920-1-RR)

CCT3 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87920-1-RR

Clone No.260042D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

TCP 1 gamma, CCT gamma, CCTG, CCTgamma, CCT-gamma

• 

  WB analysis using 87920-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87920-1-RR (CCT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 87920-1-RR

  Biolayer interferometry (BLI) kinetic assays of 87920-1-RR against Human CCT3 were performed. The affinity constant is below 1 pM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, U2OS cells, mouse brain tissue, rat brain tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87920-1-RR targets CCT3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0843

  Product name: Recombinant human CCT3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 246-545 aa of BC006501

  Sequence: EYKKGESQTDIEITREEDFTRILQMEEEYIQQLCEDIIQLKPDVVITEKGISDLAQHYLMRANITAIRRVRKTDNNRIARACGARIVSRPEELREDDVGTGAGLLEIKKIGDEYFTFITDCKDPKACTILLRGASKEILSEVERNLQDAMQVCRNVLLDPQLVPGGGASEMAVAHALTEKSKAMTGVEQWPYRAVAQALEVIPRTLIQNCGASTIRLLTSLRAKHTQENCETWGVNGETGTLVDMKELGIWEPLAVKLQTYKTAVETAVLLLRIDDIVSGHKKKGDDQSRQGGAPDAGQE

• Full Name: chaperonin containing TCP1, subunit 3 (gamma)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC006501
• Gene Symbol: CCT3
• Gene ID (NCBI): 7203
• RRID: AB_3745827
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49368
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT3 protein (full name: chaperonin containing TCP1 subunit 3, also known as the gamma subunit of TCP-1 protein) is an important member of the TCP-1 chaperonin family. As one of the eight essential subunits of the CCT/TRiC complex, its core function is to act as a molecular chaperone that assists in the correct folding of other cellular proteins under ATP hydrolysis. It is particularly involved in the folding process of key cytoskeletal components such as actin and tubulin, while also participating in important biological activities including ciliogenesis and telomere maintenance.