CCT4 Polyclonal antibody

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• CCT4 Polyclonal antibody (21524-1-AP)

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CCT4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21524-1-AP Copied!

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

CCTD, CCT-delta, Chaperonin containing T-complex polypeptide 1 subunit 4, SRB, Stimulator of TAR RNA-binding

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  WB analysis of HEK-293 using 21524-1-AP

  WB result of CCT4 antibody (21524-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT4 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 21524-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 21524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse heart using 21524-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse colon using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat colon using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells, HeLa cells, mouse heart tissue
• Positive IHC detected in: human liver cancer tissue, rat testis tissue, mouse colon tissue, mouse testis tissue, human brain tissue, human liver cancer tissue, human colon cancer tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21524-1-AP targets CCT4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16128

  Product name: Recombinant human CCT4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 108-407 aa of BC106934

  Sequence: SVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHD

• Full Name: chaperonin containing TCP1, subunit 4 (delta)
• Calculated Molecular Weight: 539 aa, 58 kDa
• Observed Molecular Weight: 58-60 kDa
• GenBank Accession Number: BC106934
• Gene Symbol: CCT4
• Gene ID (NCBI): 10575
• RRID: AB_10733520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P50991
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT4 (Chaperonin containing TCP1 complex 4), also known as TCP-1-delta, is a recent identified member of the family, that serves as a component of the chaperonin-containing TCP1 complex (TRiC) and also functions as a monomer. CCT4 has been shown to influence the cytoskeletal structure and regulate F-actin organization independently of TRiC (PMID: 41104595). CCT4 was highly expressed in human ESCC tissues and cell lines, and closely related to the poor prognosis (PMID: 36102200).

Publications

What published studies show

The CCT4 Polyclonal antibody (Cat# 21524-1-AP) has 10 citations published in Nature, Cell, Nature Communications, Molecular Cell, Science Translational Medicine, Molecular Therapy Nucleic Acids, Journal of Cell Biology, Molecular Carcinogenesis, Pathogens and Disease, and Medicine (Baltimore). Research fields span protein homeostasis and chaperonin function, aggrephagy, aggresome formation, cilia and flagella biology, muscle aging, thyroid cancer, esophageal squamous cell carcinoma, and lung adenocarcinoma.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor; Authors - Huaipeng Xing
human	WB	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma ; KD Validated
human	IF	Nat Commun; Episodic transport of protein aggregates achieves a positive size selectivity in aggresome formation; Authors - Rui Fang
mouse	WB,IHC	Mol Cell; PDCD5 promotes substrate release from the TRiC complex in cilia and flagella.; Authors - Huafang Wei
mouse	WB	Sci Transl Med; Inhibiting de novo ceramide synthesis restores mitochondrial and protein homeostasis in muscle aging; Authors - Tanes I Lima
human	WB	Mol Ther Nucleic Acids; hsa_circ_0001018 promotes papillary thyroid cancer by facilitating cell survival, invasion, G1/S cell cycle progression, and repressing cell apoptosis via crosstalk with miR-338-3p and SOX4.; Authors - Qiang Luo