CCT4 Monoclonal antibody

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  WB analysis using 67455-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67455-1-Ig (CCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of U2OS using 67455-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT4 antibody (67455-1-Ig, Clone: 3C6A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CCT4 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67455-1-Ig

Clone No.3C6A6

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rabbit, Pig and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

CCT DELTA, CCT4, Cctd, SRB, Stimulator of TAR RNA binding, TCP 1 delta

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, mouse brain tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

67455-1-Ig targets CCT4 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rabbit, Pig samples.

• Tested Reactivity: Human, Mouse, Rabbit, Pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17084

  Product name: Recombinant human CCT4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 108-407 aa of BC106934

  Sequence: SVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHD

• Full Name: chaperonin containing TCP1, subunit 4 (delta)
• Calculated Molecular Weight: 539 aa, 58 kDa
• Observed Molecular Weight: 55-58 kDa
• GenBank Accession Number: BC106934
• Gene Symbol: CCT4
• Gene ID (NCBI): 10575
• RRID: AB_2935250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P50991
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

T-complex protein 1 subunit delta (CCT4) is a component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PMID:25467444). As part of the TRiC complex, it may play a role in regulating telomere maintenance (PMID:25467444) and assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PMID:20080638). CCT4 has 2 isoforms produced by alternative splicing with the MW of 55 kDa and 58 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	J Clin Invest; ANKRD55 is a key regulator of T cell inflammation in multiple sclerosis; Authors - Chuyu Wu