Validation Data Gallery
|Positive WB detected in||COLO 320 cells, A431 cells, HEK-293 cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, mouse colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11603-1-AP targets CCT5 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CCT5 fusion protein Ag2146|
|Full Name||chaperonin containing TCP1, subunit 5 (epsilon)|
|Calculated molecular weight||541 aa, 60 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC035499|
|Gene ID (NCBI)||22948|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.
|Product Specific Protocols|
|WB protocol for CCT5 antibody 11603-1-AP||Download protocol|
|IHC protocol for CCT5 antibody 11603-1-AP||Download protocol|
|IF protocol for CCT5 antibody 11603-1-AP||Download protocol|
|IP protocol for CCT5 antibody 11603-1-AP||Download protocol|
|Click here to view our Standard Protocols|
CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.
DYX1C1 is required for axonemal dynein assembly and ciliary motility.
Proc Natl Acad Sci U S A
BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.
J Cell Biol
CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.
Front Cell Dev Biol
TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis
Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Andrea (Verified Customer) (01-03-2023)
Strong and clear band on the membrane.