CCT5 Monoclonal antibody

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  WB analysis using 67400-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67400-1-Ig (CCT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67400-1-Ig

  WB result of CCT5 antibody (67400-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT5 transfected HEK-293 cells.

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  IHC staining of human skin cancer using 67400-1-Ig

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67400-1-Ig (CCT5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 67400-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT5 antibody (67400-1-Ig, Clone: 1B12E10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 67400-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT5 antibody (67400-1-Ig, Clone: 1B12E10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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  IF Staining of U2OS using 67400-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CCT5 antibody (67400-1-Ig, Clone: 1B12E10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

CCT5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67400-1-Ig

Clone No.1B12E10

Publications(3)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1B12E10, CCT epsilon, CCTE, CCT-epsilon, Chaperonin containing T-complex polypeptide 1 subunit 5

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

67400-1-Ig targets CCT5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21421

  Product name: Recombinant human CCT5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 243-541 aa of BC035499

  Sequence: VEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRAFADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE

• Full Name: chaperonin containing TCP1, subunit 5 (epsilon)
• Calculated Molecular Weight: 541 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC035499
• Gene Symbol: CCT5
• Gene ID (NCBI): 22948
• RRID: AB_2882643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P48643
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC). CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita
human	WB	bioRxiv; Autophagosomes coordinate an AKAP11-dependent regulatory checkpoint that shapes neuronal PKA signaling; Authors - Ashley Segura-Roman
human	IF	J Clin Invest; ANKRD55 is a key regulator of T cell inflammation in multiple sclerosis; Authors - Chuyu Wu