|Positive WB detected in||HeLa cells, human brain tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15994-1-AP targets CCT7 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CCT7 fusion protein Ag8868|
|Full Name||chaperonin containing TCP1, subunit 7 (eta)|
|Calculated molecular weight||543 aa, 59 kDa|
|Observed molecular weight||59 kDa|
|GenBank accession number||BC019296|
|Gene ID (NCBI)||10574|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.
CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.
Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.
Aging (Albany NY)
Overexpression of chaperonin containing TCP1 subunit 7 has diagnostic and prognostic value for hepatocellular carcinoma.
Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.
Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.
Quantitative proteomics identified circulating biomarkers in lung adenocarcinoma diagnosis