Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody

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  FC experiment of Reh using 98116-1-RR

  1x10^6 Reh cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody (98116-1-RR, Clone: 241322E1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

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  FC experiment of human peripheral blood leukocytes using 98116-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody (98116-1-RR, Clone: 241322E1) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98116-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody (98116-1-RR, Clone:241322E1)(red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98116-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98116-1-RR against Human CD10 were performed. The affinity constant is 9.9 nM.

Neprilysin/CD10 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98116-1-RR

Clone No.241322E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

MME, Neprilysin, Enkephalinase, EC:3.4.24.11, CD10

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes, Reh cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98116-1-RR targets Neprilysin/CD10 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: membrane metallo-endopeptidase
• Calculated Molecular Weight: 750 aa, 86 kDa
• GenBank Accession Number: BC101658
• Gene Symbol: CD10
• Gene ID (NCBI): 4311
• RRID: AB_3672263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P08473
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).