Anti-Human CD100 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98345-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD100 Rabbit RecAb (98345-2-RR, Clone:242691F8) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98345-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD100 Rabbit RecAb (98345-2-RR, Clone: 242691F8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

• 

  Affinity and Kinetic Characterization of 98345-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98345-2-RR against Human CD100 were performed. The affinity constant is 0.464 nM.

CD100 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98345-2-RR

Clone No.242691F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SEMA4D, 242691F8, A8, CD 100, CD100 antigen

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98345-2-RR targets CD100 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• Calculated Molecular Weight: 96 kDa
• GenBank Accession Number: BC054500
• Gene Symbol: SEMA4D/CD100
• Gene ID (NCBI): 10507
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92854
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.