Tested Applications
| Positive FC detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 10 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| FC | See 1 publications below |
Product Information
PE-65049 targets VCAM-1/CD106 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | vascular cell adhesion molecule 1 |
| Calculated Molecular Weight | 739 aa, 81 kDa |
| GenBank Accession Number | BC017276 |
| Gene Symbol | VCAM-1 |
| Gene ID (NCBI) | 7412 |
| RRID | AB_2883848 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P19320 |
| Storage Buffer | PBS with 0.1% sodium azide and a stabilizer, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE VCAM-1/CD106 antibody PE-65049 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
