Anti-Human CD107a / LAMP1 Mouse scFv Antibody

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• Anti-Human CD107a / LAMP1 Mouse scFv Antibody (SF65596-1-MR)

CD107a / LAMP1 Recombinant Antibody for FC (Intra)

Cat No. SF65596-1-MR

Clone No.H4A3

Host / Type

Mouse / scFv

Reactivity

human

Applications

FC (Intra)

Synonyms

CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, H4A3

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  FC experiment of Jurkat using SF65596-1-MR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human CD107a / LAMP1 Mouse scFv Antibody (SF65596-1-MR, Clone: H4A3) (red) or Mouse IgG2a Isotype Control (C1.18.4) (blue), and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

SF65596-1-MR targets CD107a / LAMP1 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Type: Mouse / scFv
• Class: Recombinant
• Type: Single-chain variable fragment antibody (scFv)
• Immunogen:

  Human adherent peripheral blood cells

• Full Name: lysosomal-associated membrane protein 1
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC006345
• Gene Symbol: LAMP1
• Gene ID (NCBI): 3916
• ENSEMBL Gene ID: ENSG00000185896
• RRID: AB_3750141
• Conjugate: Unconjugated
• Source: Anti-Human CD107a / LAMP1 scFv from clone H4A3, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus.
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin 300, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).