Tested Applications
| Positive WB detected in | NIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32148-1-AP targets CD107b / LAMP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg2029 Product name: Recombinant Mouse CD107b/LAMP2 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 26-379 aa of NM_001017959.2 Sequence: LIVNLTDSKGTCLYAEWEMNFTITYETTNQTNKTITIAVPDKATHDGSSCGDDRNSAKIMIQFGFAVSWAVNFTKEASHYSIHDIVLSYNTSDSTVFPGAVAKGVHTVKNPENFKVPLDVIFKCNSVLTYNLTPVVQKYWGIHLQAFVQNGTVSKNEQVCEEDQTPTTVAPIIHTTAPSTTTTLTPTSTPTPTPTPTPTVGNYSIRNGNTTCLLATMGLQLNITEEKVPFIFNINPATTNFTGSCQPQSAQLRLNNSQIKYLDFIFAVKNEKRFYLKEVNVYMYLANGSAFNISNKNLSFWDAPLGSSYMCNKEQVLSVSRAFQINTFNLKVQPFNVTKGQYSTAQDCSADEDN Predict reactive species |
| Full Name | lysosomal-associated membrane protein 2 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 70-100 kDa |
| GenBank Accession Number | NM_001017959.2 |
| Gene Symbol | CD107b / LAMP2 |
| Gene ID (NCBI) | 16784 |
| RRID | AB_3670208 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P17047-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP2 is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD107b / LAMP2 antibody 32148-1-AP | Download protocol |
| IHC protocol for CD107b / LAMP2 antibody 32148-1-AP | Download protocol |
| WB protocol for CD107b / LAMP2 antibody 32148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CD107b/LAMP2 polyclonal antibody (Cat# 32148-1-AP) has 7 total citations. Citations appear in Frontiers in Immunology, Ecotoxicology and Environmental Safety, International Journal of Molecular Sciences, Glia, Cell Signaling, BBA Molecular Cell Research, and Gut Microbes. Research fields span cancer immunotherapy, autophagy and lysosomal biology, obesity-related cardiomyopathy, Parkinson's disease, bronchopulmonary dysplasia, and intestinal inflammation.
| Species | Application | Title |
|---|---|---|
Front Immunol A bifunctional peptide-selenium nanocomposite for lysosomal degradation of PD-L1 and enhanced cancer immunotherapy. | ||
Ecotoxicol Environ Saf Fluoride exposure impairs lysosomal biogenesis and autophagic flux in myocardial injury: Involvement of the FNIP1/mTOR/TFEB pathway. | ||
Int J Mol Sci Dysfunction of Autophagy in Adipose Tissue Macrophages Regulated via FoxO1 in Obesity-Related Severe Acute Pancreatitis. | ||
Glia VPS35 Regulates Microglial Lipid Droplet Accumulation in Parkinson's Disease via Rab7. | ||
Cell Signal Mst1 inhibition attenuates obesity cardiomyopathy via reversing FUNDC1-related mitophagy. | ||
Biochim Biophys Acta Mol Cell Res SFRP2 is involved in hyperoxia-induced bronchopulmonary dysplasia in newborn mice by suppressing Nrf2/HO-1 pathway. |
