Multi-rAb® CD107b / LAMP2 Multi-Recombinant antibody

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• Multi-rAb® CD107b / LAMP2 Multi-Recombinant antibody (RMX00094)

CD107b / LAMP2 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00094

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD107b, LAMP2, CD107 antigen-like family member B, LAMP 2, LAMP-2

• 

  WB analysis using RMX00094

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00094 (CD107b / LAMP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using RMX00094

  WB result of CD107b / LAMP2 antibody (RMX00094; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD107b / LAMP2 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using RMX00094

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using RMX00094 (CD107b / LAMP2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using RMX00094

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using RMX00094 (CD107b / LAMP2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using RMX00094

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CD107b / LAMP2 antibody (RMX00094) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00094 targets CD107b / LAMP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  FusionProtein

• Full Name: lysosomal-associated membrane protein 2
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 100 kDa~ 120 kDa
• GenBank Accession Number: NM_002294.3
• Gene Symbol: LAMP2
• Gene ID (NCBI): 3920
• ENSEMBL Gene ID: ENSG00000005893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P13473-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides.