CoraLite®647-conjugated Anti-Mouse CD117 (c-Kit) (ACK2)

CD117 (c-Kit) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b

Reactivity

Mouse

Applications

FC, ELISA

Conjugate

CoraLite®647 Fluorescent Dye

CloneNo.

ACK2

Cat no : CL647-65094

Synonyms

Bs, c KIT, CD117, Fdc, Gsfsco1, Gsfsco5, Gsfsow3, Kit, kit oncogene, SCO1, SCO5, SOW3, Ssm, Tr kit



Tested Applications

Positive FC detected inmouse bone marrow cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65094 targets CD117 (c-Kit) in FC, ELISA applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b
Class Monoclonal
Type Antibody
Immunogen Murine IL-3 dependent mast cells
Full Name kit oncogene
GenBank accession numberBC052457
Gene symbol Kit
Gene ID (NCBI) 16590
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

Protocols

Product Specific Protocols
FC protocol for CL647-conjugated CD117 (c-Kit) antibody CL647-65094Download protocol
Standard Protocols
Click here to view our Standard Protocols