CoraLite® Plus 647 Anti-Mouse CD117 (c-Kit) (ACK2)

CD117 (c-Kit) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

ACK2

Cat no : CL647-65094

Synonyms

Bs, c KIT, CD117, CD117 (c-Kit), Fdc, Gsfsco1, Gsfsco5, Gsfsow3, Kit, kit oncogene, SCO1, SCO5, SOW3, Ssm, Tr kit

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Validation Data Gallery

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  FC experiment of mouse bone marrow cells using CL647-65094

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC-Anti-Mouse CD11b (FITC-65055, Clone: M1/70) and 5.00 ul CoraLite® Plus 647-conjugated Anti-Mouse CD117 (c-Kit) (CL647-65094, Clone: ACK2). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using CL647-65094

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC-Anti-Mouse CD11b (FITC-65055, Clone: M1/70) and CoraLite® Plus 647-conjugated Rat IgG2b isotype control antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65094 targets CD117 (c-Kit) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Murine IL-3 dependent mast cells
• Full Name: kit oncogene
• GenBank Accession Number: BC052457
• Gene Symbol: CD117
• Gene ID (NCBI): 16590
• RRID: AB_2883678
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).