CD11B/Integrin Alpha M Polyclonal antibody

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  IHC staining of human tonsillitis using 20991-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20991-1-AP (CD11B/Integrin alpha M Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 20991-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20991-1-AP (CD11B/Integrin alpha M Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 20991-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20991-1-AP (CD11B/Integrin alpha M Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 20991-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20991-1-AP (CD11B/Integrin alpha M Antibody) at dilution of 1:200 (under 40x lens).

CD11B/Integrin Alpha M Polyclonal Antibody for ELISA

Cat No. 20991-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

CD11B, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6

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Published Applications

• WB: See 15 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Product Information

20991-1-AP targets CD11B/Integrin Alpha M in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: integrin, alpha M (complement component 3 receptor 3 subunit)
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 110-170 kDa
• GenBank Accession Number: NM_000632
• Gene Symbol: CD11b
• Gene ID (NCBI): 3684
• ENSEMBL Gene ID: ENSG00000169896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11215
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publications

Species	Application	Title
	IHC	J Nanobiotechnology; Synergy of nanodiamond-doxorubicin conjugates and PD-L1 blockade effectively turns tumor-associated macrophages against tumor cells; Authors - Hua‑Zhen Xu
human	IHC	Cancer Lett; Piribedil disrupts the MLL1-WDR5 interaction and sensitizes MLL-rearranged acute myeloid leukemia (AML) to doxorubicin-induced apoptosis.; Authors - Xiong Zhang
human	WB	Cancer Immunol Immunother; Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.; Authors - Pei Yang
	IF	J Nanobiotechnology; Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.; Authors - Shen-Jun Yuan
	IHC	Neuropharmacology; Trans-cinnamaldehyde improves memory impairment by blocking microglial activation through the destabilization of iNOS mRNA in mice challenged with lipopolysaccharide.; Authors - Liqing Zhang
	WB,IHC	Mol Neurobiol; Myeloid TBK1 Deficiency Induces Motor Deficits and Axon Degeneration Through Inflammatory Cell Infiltration.; Authors - Weisong Duan