Anti-Human CD11b Rabbit Recombinant Antibody

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• Anti-Human CD11b Rabbit Recombinant Antibody (98785-2-RR)

CD11b Uni-rAb^® Recombinant Antibody for FC

Cat No. 98785-2-RR

Clone No.252751B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD11 antigen-like family member B, Cell surface glycoprotein MAC-1 subunit alpha, CR 3 alpha chain, CR-3 alpha chain, CR3A

• 

  FC experiment of human PBMCs using 98785-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ITAM Rabbit RecAb (98785-2-RR, Clone:252751B5) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 405 Anti-Human CD14 Rabbit Recombinant Antibody (CL405-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using 98785-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ITAM Rabbit RecAb (98785-2-RR, Clone:252751B5) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98785-2-RR targets CD11b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10485

  Product name: Recombinant human ITAM protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011430 of

  Sequence: \

• Full Name: integrin, alpha M (complement component 3 receptor 3 subunit)
• Gene Symbol: CD11b
• Gene ID (NCBI): 3684
• ENSEMBL Gene ID: ENSG00000169896
• RRID: AB_3746346
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).