FC experiment of mouse bone marrow cells using Biotin-65055
1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Biotin Anti-Mouse CD11b (M1/70) (Biotin-65055, Clone: M1/70) and SA-PE, or 0.5 ug Isotype Control. Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Biotin Anti-Mouse CD11b (M1/70) (Biotin-65055, Clone: M1/70) and SA-PE, or 0.5 ug Isotype Control. Cells were not fixed.
FC experiment of mouse bone marrow cells using Biotin-65055
1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Biotin Anti-Mouse CD11b (M1/70) (Biotin-65055, Clone: M1/70) and SA-PE, or 0.5 ug Isotype Control. Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Biotin Anti-Mouse CD11b (M1/70) (Biotin-65055, Clone: M1/70) and SA-PE, or 0.5 ug Isotype Control. Cells were not fixed.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
Biotin-65055 targets CD11b in FC, ELISA applications and shows reactivity with human, mouse, monkey, guinea pig, non-human primates samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocols
Product Specific Protocols
FC protocol for Biotin CD11b antibody Biotin-65055
FC experiment of mouse bone marrow cells using Biotin-65055
1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Biotin Anti-Mouse CD11b (M1/70) (Biotin-65055, Clone: M1/70) and SA-PE, or 0.5 ug Isotype Control. Cells were not fixed.
FC experiment of mouse bone marrow cells using Biotin-65055
1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Biotin Anti-Mouse CD11b (M1/70) (Biotin-65055, Clone: M1/70) and SA-PE, or 0.5 ug Isotype Control. Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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