CoraLite® Plus 405 Anti-Mouse CD11b (M1/70)

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  FC experiment of mouse bone marrow cells using CL405-65055

  1x10^6 mouse bone marrow cells were surface stained with CoraLite® Plus 647 Anti-Mouse Ly-6G/Ly-6C, and 0.25 ug CoraLite® Plus 405 Anti-Mouse CD11b (M1/70) (CL405-65055, Clone: M1/70) or CoraLite® Plus 405 Rat IgG2b Isotype Control (LTF-2) (CL405-65211, Clone: LTF-2). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using CL405-65055

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 405 Anti-Mouse CD11b (M1/70) (CL405-65055, Clone: M1/70) (red) or CoraLite® Plus 405 Rat IgG2b Isotype Control (LTF-2) (CL405-65211, Clone: LTF-2) (blue). Cells were not fixed.

CD11b Monoclonal Antibody for FC

Cat No. CL405-65055

Clone No.M1/70

Host / Isotype

Rat / IgG2b, kappa

Reactivity

human, mouse

Applications

FC

Synonyms

CR3, CR3A, integrin alpha M, Itgam, Ly 40

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65055 targets CD11b in FC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes
• Full Name: integrin alpha M
• Calculated Molecular Weight: 127 kDa
• GenBank Accession Number: BC156991
• Gene Symbol: CD11b
• Gene ID (NCBI): 16409
• RRID: AB_3083819
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: E9Q604
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).