Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD11b (CL488-65055, Clone: M1/70) at dilution of 1:400.
FC experiment of mouse bone marrow cells using CL488-65055
1x10^6 mouse bone marrow cells were surface stained with APC Anti-Mouse Ly-6G/Ly-6C (Gr-1), and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11b (M1/70) (CL488-65055, Clone: M1/70) or CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2). Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with APC Anti-Mouse Ly-6G/Ly-6C (Gr-1), and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11b (M1/70) (CL488-65055, Clone: M1/70) or CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2). Cells were not fixed.
FC experiment of mouse bone marrow cells using CL488-65055
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11b (M1/70) (CL488-65055, Clone:M1/70) (red) or CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2) (blue). Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11b (M1/70) (CL488-65055, Clone:M1/70) (red) or CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2) (blue). Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocols
Product Specific Protocols
FC protocol for CL Plus 488 CD11b antibody CL488-65055
Mitochondrion-NLRP3 inflammasome activation in macrophages: A novel mechanism of the anti-inflammatory effect of Notopterygium in rheumatoid arthritis treatment
FC experiment of mouse bone marrow cells using CL488-65055
1x10^6 mouse bone marrow cells were surface stained with APC Anti-Mouse Ly-6G/Ly-6C (Gr-1), and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11b (M1/70) (CL488-65055, Clone: M1/70) or CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2). Cells were not fixed.
FC experiment of mouse bone marrow cells using CL488-65055
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11b (M1/70) (CL488-65055, Clone:M1/70) (red) or CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2) (blue). Cells were not fixed.
IF/ICC Figures
IF Staining of BV-2 using CL488-65055
Immunofluorescent analysis of (4% PFA) fixed BV-2 cells using CoraLite® Plus 488 CD11b antibody (CL488-65055, Clone: M1/70 ) at dilution of 1:400.
IF Staining of mouse splenocytes using CL488-65055
Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD11b (CL488-65055, Clone: M1/70) at dilution of 1:400.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
