CoraLite®488 Anti-Mouse CD11b (M1/70)

CD11b Monoclonal Antibody for IF,FC

Host / Isotype

Rat / IgG2b

Reactivity

Mouse

Applications

IF,FC

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

M1/70

Cat no : CL488-65055

Synonyms

Cd11b, CD11b/CD18, CR3, CR3A, F730045J24Rik, integrin alpha M, Itgam, Ly 40, Mac 1, Mac 1a, MAC1



Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse bone marrow cells
For other applications, we recommend the unconjugated version of this antibody, 65055-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65055 targets CD11b in IF,FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b
Class Monoclonal
Type Antibody
Immunogen C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes
Full Name integrin alpha M
GenBank accession numberBC156991
Gene symbol Itgam
Gene ID (NCBI) 16409
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Product Specific Protocols
IF protocol for CL488 CD11b antibody CL488-65055Download protocol
FC protocol for CL488 CD11b antibody CL488-65055Download protocol
Standard Protocols
Click here to view our Standard Protocols