CoraLite®594 Anti-Mouse CD11b (M1/70)

CD11b Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse, Human

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

M1/70

Cat no : CL594-65055

Synonyms

Cd11b, CD11b/CD18, CR3, CR3A, F730045J24Rik, integrin alpha M, Itgam, Ly 40, Mac 1, Mac 1a, MAC1

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Validation Data Gallery

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  FC experiment of mouse bone marrow cells using CL594-65055

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with 5 ul CoraLite®594-conjugated Anti-Mouse CD11b (CL594-65055, Clone: M1/70) (red) or isotype control antibody (black). Cells were not fixed .

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65055 targets CD11b in FC applications and shows reactivity with Mouse, Human samples.

• Tested Reactivity: Mouse, Human
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes
• Full Name: integrin alpha M
• GenBank Accession Number: BC156991
• Gene Symbol: Itgam
• Gene ID (NCBI): 16409
• RRID: AB_2919927
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).