PerCP-Cyanine5.5 Anti-Mouse CD11b (M1/70)

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  FC experiment of mouse bone marrow cells using CPY5-65055

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug PerCP-Cyanine5.5 Anti-Mouse CD11b (M1/70) (CPY5-65055, Clone:M1/70) or unstained. Cells were then stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (CL647-65140, Clone: RB6-8C5). Cells were not fixed.

CD11b Monoclonal Antibody for FC

Cat No. CPY5-65055

Clone No.M1/70

Host / Isotype

Rat / IgG2b, kappa

Reactivity

human, mouse, guinea pig

Applications

FC

Synonyms

CR3, CR3A, integrin alpha M, Itgam, Ly 40

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CPY5-65055 targets CD11b in FC applications and shows reactivity with human, mouse, guinea pig samples.

• Tested Reactivity: human, mouse, guinea pig
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes

• Full Name: integrin alpha M
• Calculated Molecular Weight: 127 kDa
• GenBank Accession Number: BC156991
• Gene Symbol: CD11b
• Gene ID (NCBI): 16409
• RRID: AB_3673816
• Conjugate: PerCP-Cyanine5.5 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 482 nm / 690 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: E9Q604
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).