Tested Applications
| Positive FC detected in | mouse bone marrow cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CPY5-FcA65672 targets CD11b in FC applications and shows reactivity with human, mouse, rabbit, cynomolgus monkey, guinea pig samples.
| Tested Reactivity | human, mouse, rabbit, cynomolgus monkey, guinea pig |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | integrin alpha M |
| Calculated Molecular Weight | 127 kDa |
| GenBank Accession Number | BC156991 |
| Gene Symbol | CD11b |
| Gene ID (NCBI) | 16409 |
| RRID | AB_3749224 |
| Conjugate | PerCP-Cyanine5.5 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 482 nm / 690nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | E9Q604 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PerCP-Cyanine5.5 CD11b antibody CPY5-FcA65672 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
FcZero-rAb® Anti-Mouse CD11b (M1/70) Rabbit Recombinant Antibody (Cat# CPY5-FcA65672) has 1 citation. It appears in J Biochem Mol Toxicol, a study investigating UCP2-mediated mitochondrial metabolic reprogramming in glioblastoma cells and its role in repolarizing tumor-associated macrophages for immunotherapy. The associated research fields include neuro-oncology, mitochondrial metabolism, macrophage biology, and cancer immunotherapy.
| Species | Application | Title |
|---|---|---|
J Biochem Mol Toxicol UCP2 in Glioblastoma Cells Regulates Mitochondrial Metabolic Reprogramming to Mediate Immunophenotypic Repolarization of Tumor-Associated Macrophages and Immunotherapy. |
