PE-Cyanine7 Anti-Mouse CD11c (N418) Mouse IgG2a Recombinant Antibody

CD11c Uni-rAbTM Recombinant Antibody for FC
Cat No. PY7-65602
Clone No.N418

Host / Isotype

Mouse / IgG2a

Reactivity

mouse

Applications

FC

Itgax, Cr4, integrin alpha X, N418

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PY7-65602 targets CD11c in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Mouse spleen dendritic cells Predict reactive species
Full Name integrin alpha X
GenBank Accession NumberBC167225
Gene Symbol CD11c
Gene ID (NCBI) 16411
Conjugate PE-Cyanine7 Fluorescent Dye
Excitation/Emission Maxima Wavelengths450-500 nm / 778 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

Protocols

Product Specific Protocols
FC protocol for PE-Cyanine7 CD11c antibody PY7-65602Download protocol
Standard Protocols
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