PE-Cyanine7 Anti-Mouse CD11c (N418) Mouse IgG2a Recombinant Antibody

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  FC experiment of mouse splenocytes using PY7-65602

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2), and 0.25 ug PE-Cyanine7 Anti-Mouse CD11c (N418) Mouse IgG2a RecAb (PY7-65602, Clone: N418) or unstained. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PY7-65602

  1x10^6 mouse splenocytes were surface stained with 0.25 ug PE-Cyanine7 Anti-Mouse CD11c (N418) Mouse IgG2a RecAb (PY7-65602, Clone: N418) (red) or unstained (blue). Cells were not fixed.

CD11c Uni-rAb^TM Recombinant Antibody for FC

Cat No. PY7-65602

Clone No.N418

Host / Isotype

Mouse / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

Itgax, Cr4, integrin alpha X, N418

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PY7-65602 targets CD11c in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Mouse spleen dendritic cells
• Full Name: integrin alpha X
• GenBank Accession Number: BC167225
• Gene Symbol: CD11c
• Gene ID (NCBI): 16411
• Conjugate: PE-Cyanine7 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 450-500 nm / 778 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).