Home
Products
Primary Antibodies
Flow Cytometry Antibodies
Anti-Human CD13 Rabbit Recombinant Antibody, PBS Only (98577-1-PBS)

Anti-Human CD13 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human CD13 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98577-1-PBS-1MG

CD13 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98577-1-PBS

Clone No.251501H7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP-M

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllFCAffinity

FC experiment of human peripheral blood leukocytes using 98577-1-RR (same clone as 98577-1-PBS)
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD13 Rabbit RecAb (98577-1-RR, Clone: 251501H7) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98577-1-PBS in a different storage buffer formulation.
×
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD13 Rabbit RecAb (98577-1-RR, Clone: 251501H7) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98577-1-PBS in a different storage buffer formulation.
FC experiment of human peripheral blood leukocytes using 98577-1-RR (same clone as 98577-1-PBS)
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD13 Rabbit RecAb (98577-1-RR, Clone: 251501H7) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98577-1-PBS in a different storage buffer formulation.
×
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD13 Rabbit RecAb (98577-1-RR, Clone: 251501H7) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98577-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 98577-1-RR
Biolayer interferometry (BLl) kinetic assays of 98577-1-RR against Human CD13 were performed. The affinity constant is 1.60 nM.

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

98577-1-PBS targets CD13 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg3748 Product name: Recombinant Human CD13 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 69-967 aa of BC058928 Sequence: KAWNRYRLPNTLKPDSYRVTLRPYLTPNDRGLYVFKGSSTVRFTCKEATDVIIIHSKKLNYTLSQGHRVVLRGVGGSQPPDIDKTELVEPTEYLVVHLKGSLVKDSQYEMDSEFEGELADDLAGFYRSEYMEGNVRKVVATTQMQAADARKSFPCFDEPAMKAEFNITLIHPKDLTALSNMLPKGPSTPLPEDPNWNVTEFHTTPKMSTYLLAFIVSEFDYVEKQASNGVLIRIWARPSAIAAGHGDYALNVTGPILNFFAGHYDTPYPLPKSDQIGLPDFNAGAMENWGLVTYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNLVTIEWWNDLWLNEGFASYVEYLGADYAEPTWNLKDLMVLNDVYRVMAVDALASSHPLSTPASEINTPAQISELFDAISYSKGASVLRMLSSFLSEDVFKQGLASYLHTFAYQNTIYLNLWDHLQEAVNNRSIQLPTTVRDIMNRWTLQMGFPVITVDTSTGTLSQEHFLLDPDSNVTRPSEFNYVWIVPITSIRDGRQQQDYWLIDVRAQNDLFSTSGNEWVLLNLNVTGYYRVNYDEENWRKIQTQLQRDHSAIPVINRAQIINDAFNLASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKNYLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWMENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWILNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQWFTENSK Predict reactive species
Full Name	alanyl (membrane) aminopeptidase
Calculated Molecular Weight	110 kDa
GenBank Accession Number	BC058928
Gene Symbol	CD13
Gene ID (NCBI)	290
ENSEMBL Gene ID	ENSG00000166825
RRID	AB_3746190
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P15144
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane protein expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)