PE Anti-Human CD137 (4B4-1)

CD137 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

4B4-1

Cat no : PE-65159

Synonyms

4 1BB, 4 1BB ligand receptor, CD137, CDw137, ILA, T cell antigen 4 1BB homolog, T cell antigen ILA, TNFRSF9

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Validation Data Gallery

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  FC experiment of human peripheral blood mononuclear cells using PE-65159

  1X10^6 PHA-stimulated (5 μg/mL, 3 days) (red) or unstimulated (blue) human peripheral blood mononuclear cells (PBMCs) were surface stained with 10.00 ul PE Anti-Human CD137 (PE-65159, Clone: 4B4-1). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human peripheral blood mononuclear cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65159 targets CD137 in FC applications and shows reactivity with Human, Non-human primates samples.

• Tested Reactivity: Human, Non-human primates
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant human CD137 ectodomain
• Full Name: tumor necrosis factor receptor superfamily, member 9
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: BC006196
• Gene Symbol: TNFRSF9
• Gene ID (NCBI): 3604
• RRID: AB_2883937
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.