CD138/Syndecan-1 Monoclonal antibody

CD138/Syndecan-1 Monoclonal Antibody for IF, IHC,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

4F3A8

Cat no : 67155-1-Ig

Synonyms

CD138, SDC, SDC1, SYND1, syndecan, syndecan 1

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Validation Data Gallery

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  IHC staining of human tonsillitis using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (SDC1,CD138 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (SDC1,CD138 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 67155-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 67155-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue, human ovary tumor tissue, human appendicitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

67155-1-Ig targets CD138/Syndecan-1 in IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD138/Syndecan-1 fusion protein Ag0823
• Full Name: syndecan 1
• Calculated Molecular Weight: 32.5 kDa
• GenBank Accession Number: BC008765
• Gene Symbol: SDC1
• Gene ID (NCBI): 6382
• RRID: AB_2882452
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.

Publications

Species	Application	Title
human	IF	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	IF	Nat Commun; Establishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection; Authors - Pei-Fen Zhang