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Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody, PBS Only (65616-1-PBS)

Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody, PBS Only

Name: Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 65616-1-PBS-1MG

CD14 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65616-1-PBS

Clone No.26IC

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

Synonyms

26IC, CD14 molecule, Monocyte differentiation antigen CD14, membrane-bound form, Monocyte differentiation antigen CD14, urinary form, Myeloid cell-specific leucine-rich glycoprotein

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Filter: AllFC

FC experiment of human PBMCs using 65616-1-MR (same clone as 65616-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody (65616-1-MR, Clone: 26IC) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65616-1-PBS in a different storage buffer formulation.
×
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody (65616-1-MR, Clone: 26IC) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65616-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65616-1-MR (same clone as 65616-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody (65616-1-MR, Clone:26IC) (red) or Mouse IgG2a Isotype Control (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65616-1-PBS in a different storage buffer formulation.
×
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 (26IC) Mouse IgG2a Recombinant Antibody (65616-1-MR, Clone:26IC) (red) or Mouse IgG2a Isotype Control (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65616-1-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

65616-1-PBS targets CD14 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Recombinant
Type	Antibody
Immunogen	N/A Predict reactive species
Full Name	CD14 molecule
Calculated Molecular Weight	375 aa, 40 kDa
GenBank Accession Number	BC010507
Gene Symbol	CD14
Gene ID (NCBI)	929
ENSEMBL Gene ID	ENSG00000170458
RRID	AB_3670362
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P08571
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.