CD147 Polyclonal antibody

• 

  WB analysis using 32187-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32187-1-AP (CD147 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 32187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 32187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 32187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 32187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD147 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32187-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

Basic immunoglobulin superfamily, Basigin, Bsg, HT7 antigen, Membrane glycoprotein gp42

---

Tested Applications

• Positive WB detected in: RAW 264.7 cells, mouse small intestine tissue, mouse spleen tissue, mouse thymus tissue
• Positive IHC detected in: mouse small intestine tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32187-1-AP targets CD147 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: basigin
• Calculated Molecular Weight: 42kDa
• Observed Molecular Weight: 35-55 kDa
• GenBank Accession Number: NM_009768.2
• Gene Symbol: CD147
• Gene ID (NCBI): 12215
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P18572-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion, and a single transmembrane region. CD147 is expressed in various cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte, and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported thatthe spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020).