Tested Applications
| Positive WB detected in | TF-1 cells, human spleen tissue, Jurkat cells, PNGF treated TF-1 cells, MOLT-4 cell |
| Positive IHC detected in | human liver cancer tissue, human placenta tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | A431 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66443-1-Ig targets CD147 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2619 Product name: Recombinant human CD147 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-269 aa of BC009040 Sequence: MAAALFVLLGFALLGTHGASGAAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALWPFLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS Predict reactive species |
| Full Name | basigin (Ok blood group) |
| Calculated Molecular Weight | 385 aa, 42 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC009040 |
| Gene Symbol | CD147 |
| Gene ID (NCBI) | 682 |
| RRID | AB_2881813 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P35613 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD147 antibody 66443-1-Ig | Download protocol |
| IHC protocol for CD147 antibody 66443-1-Ig | Download protocol |
| WB protocol for CD147 antibody 66443-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-CD147 antibody (Cat# 66443-1-Ig) has 12 citations published in journals including Nature Communications, Advanced Science, Cancer Biology & Medicine, Journal of Cell Biology, Cancer Science, Scientific Reports, BMC Cancer, and others. Research fields span exosome biology, rheumatoid arthritis, tumor immune microenvironment, targeted drug delivery, viral infections (SARS-CoV-2, influenza A), cervical cancer, colorectal cancer, pulmonary fibrosis, protein glycosylation, and breast cancer prognosis.
| Species | Application | Title |
|---|---|---|
Nat Commun Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. | ||
Adv Sci (Weinh) Inhibition of Aberrant Activated Fibroblast-Like Synoviocytes in Rheumatoid Arthritis by Leishmania Peptide via the Regulation of Fatty Acid Synthesis Metabolism | ||
Cancer Biol Med SARS-CoV-2 infection in brain tumors and the association with alterations in the tumor immune microenvironment. | ||
Colloids Surf B Biointerfaces Dextran-doxorubicin prodrug nanoparticles conjugated with CD147 monoclonal antibody for targeted drug delivery in hepatoma therapy | ||
Front Microbiol Cyclophilin A causes severe fever with thrombocytopenia syndrome virus-induced cytokine storm by regulating mitogen-activated protein kinase pathway |
Reviews
What customers say
The single review for 66443-1-Ig was posted by Guorong Sun on March 31, 2022. The reviewer used the antibody for Western Blot at a 1:5000 dilution on ARPE-19 cells (lot 10004516) and reported that it did not perform well with this cell line, giving it a rating of 3 out of 5. No additional details or troubleshooting notes were provided.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Guorong (Verified Customer) (03-31-2022) | Seems not good for ARPE-19 cells
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