|Positive WB detected in||TF-1 cells, MOLT-4 cells, human spleen tissue, Jurkat cells|
|Positive IHC detected in||human liver cancer tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
66443-1-Ig targets CD147 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, pig samples.
|Tested Reactivity||human, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD147 fusion protein Ag2619|
|Full Name||basigin (Ok blood group)|
|Calculated molecular weight||385 aa, 42 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC009040|
|Gene ID (NCBI)||682|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion and an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).
|Product Specific Protocols|
|WB protocol for CD147 antibody 66443-1-Ig||Download protocol|
|IHC protocol for CD147 antibody 66443-1-Ig||Download protocol|
|IF protocol for CD147 antibody 66443-1-Ig||Download protocol|
|FC protocol for CD147 antibody 66443-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Anal Bioanal Chem
Novel gene-encoded intermolecular FRET sensor for tracking glycosylation of CD147 in living cells.
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