Validation Data Gallery
|Positive WB detected in
|TF-1 cells, human spleen tissue, MOLT-4 cell, Jurkat cells
|Positive IHC detected in
|human liver cancer tissue, human placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|human tonsillitis tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IHC : 1:50-1:500
|IF : 1:400-1:1600
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66443-1-Ig targets CD147 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, pig samples.
|Host / Isotype
|Mouse / IgG1
|CD147 fusion protein Ag2619
|basigin (Ok blood group)
|Calculated molecular weight
|385 aa, 42 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).
|Product Specific Protocols
|WB protocol for CD147 antibody 66443-1-Ig
|IHC protocol for CD147 antibody 66443-1-Ig
|IF protocol for CD147 antibody 66443-1-Ig
|FC protocol for CD147 antibody 66443-1-Ig
|Click here to view our Standard Protocols
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9.
HSP90 promotes radioresistance of cervical cancer cells via reducing FBXO6-mediated CD147 polyubiquitination.
Biochim Biophys Acta Mol Cell Res
Crosstalk between pleural mesothelial cell and lung fibroblast contributes to pulmonary fibrosis.
Anal Bioanal Chem
Novel gene-encoded intermolecular FRET sensor for tracking glycosylation of CD147 in living cells.
Cyclophilin A causes severe fever with thrombocytopenia syndrome virus-induced cytokine storm by regulating mitogen-activated protein kinase pathway
Colloids Surf B Biointerfaces
Dextran-doxorubicin prodrug nanoparticles conjugated with CD147 monoclonal antibody for targeted drug delivery in hepatoma therapy
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guorong (Verified Customer) (03-31-2022)
Seems not good for ARPE-19 cells