CD147 Antibody

CD147 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1G12B5

Cat no : 66443-1-Ig

Synonyms

5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF



Tested Applications

Positive WB detected inJurkat cells, TF-1 cells, MOLT-4 cells, human spleen tissue
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66443-1-Ig targets CD147 in WB, IHC, FC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD147 fusion protein Ag2619
Full Name basigin (Ok blood group)
Calculated molecular weight 385aa,42 kDa
Observed molecular weight 45-50 kDa
GenBank accession number BC009040
Gene symbol BSG
Gene ID (NCBI) 682
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BSG, also named as 5F7, Basigin, EMMPRIN, TCSF and CD147, plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS).CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. It seems to be a receptor for oligomannosidic glycans. CD147 is a receptor of CypA, inducing matrix metalloproteinase expression and mediating the degradation of the extracellular matrix, plays an important role in tumorigenesis and invasion in oral cancer. It has been considered as an objective and effective marker to predict invasion and prognosis in various cancers.

Protocols

Product Specific Protocols
WB protocol for CD147 antibody 66443-1-IgDownload protocol
IHC protocol for CD147 antibody 66443-1-IgDownload protocol
FC protocol for CD147 antibody 66443-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols