Anti-Human CD15 Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of human peripheral blood leukocytes using 98616-1-RR (same clone as 98616-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD15 Rabbit RecAb (98616-1-RR, Clone: 252342A1) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98616-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98616-1-RR (same clone as 98616-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD15 Rabbit RecAb (98616-1-RR, Clone: 252342A1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98616-1-PBS in a different storage buffer formulation.

CD15 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98616-1-PBS

Clone No.252342A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Lewis X, SSEA-1

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Product Information

98616-1-PBS targets CD15 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: CD15
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD15, also known as stage-specific embryonic antigen-1 (SSEA-1) or Lewis X (LeX), is a cell surface glycan consisting of a trisaccharide with the structure Galβ1-4[Fucα1-3]GlcNAc (PMID: 18953356). CD15 is expressed on most terminally differentiated myeloid cells, including neutrophils, eosinophils, monocytes, macrophages, mast cells, and Langerhans cells. It has also been shown to be expressed on stem cells and progenitors in the embryonic and adult nervous system, Hodgkin's lymphoma cells, and cells from a variety of solid cancers (PMID: 19185848). CD15 acts as a ligand for selectins in transendothelial migration and is involved in phagocytosis and chemotaxis (PMID: 1362195).