Anti-Human CD155/PVR (SKII.4)

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  FC experiment of U937 using 65251-1-Ig

  1X10^6 U937 cells were surface stained with 0.2 ug Anti-Human CD155/PVR (65251-1-Ig, Clone:SKII.4) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.

CD155/PVR Monoclonal Antibody for FC

Cat No. 65251-1-Ig

Clone No.SKII.4

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

CD155, FLJ25946, HVED, Necl 5, NECL5, Nectin like protein 5, poliovirus receptor, PVR, PVS, TAGE4

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Tested Applications

• Positive FC detected in: U937

Recommended dilution

• Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65251-1-Ig targets CD155/PVR in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: SK-N-AS human neuroblastoma cells
• Full Name: poliovirus receptor
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC015542
• Gene Symbol: CD155/PVR
• Gene ID (NCBI): 5817
• RRID: AB_2935152
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P15151
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.