Mouse CD157 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84417-5-RR (same clone as 84417-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84417-5-RR (CD157 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84417-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84417-5-RR (same clone as 84417-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84417-5-RR (CD157 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84417-5-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 84417-5-RR (same clone as 84417-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD157 antibody (84417-5-RR, Clone: 241822F10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84417-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84417-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84417-5-RR against Mouse CD157 were performed. The affinity constant is 5.36 nM.

CD157 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84417-5-PBS

Clone No.241822F10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Bp3, Bone marrow stromal antigen 1, Antigen BP3, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2

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Product Information

84417-5-PBS targets CD157 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: bone marrow stromal cell antigen 1
• Calculated Molecular Weight: 35kDa
• Observed Molecular Weight: 40-46 kDa
• Gene Symbol: Bst1
• Gene ID (NCBI): 12182
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q64277
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD157 is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores. CD157 has a calculated molecular mass of 30-36 kDa and an apparent molecular mass of 40-46 kDa due to glycosylation (PMID: 8941363, 10491089).