Anti-Mouse CD157 Rabbit Recombinant Antibody, PBS Only

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• Anti-Mouse CD157 Rabbit Recombinant Antibody, PBS Only (98213-1-PBS)

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  FC experiment of mouse splenocytes using 98213-1-RR (same clone as 98213-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD157 Rabbit Recombinant Antibody (98213-1-RR, Clone: 241822H1) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed. This data was developed using the same antibody clone with 98213-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98213-1-RR (same clone as 98213-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD157 Rabbit Recombinant Antibody (98213-1-RR, Clone: 241822H1) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98213-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98213-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98213-1-RR against Mouse CD157 were performed. The affinity constant is 0.904 nM.

CD157 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98213-1-PBS

Clone No.241822H1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Bp3, Bone marrow stromal antigen 1, Antigen BP3, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2

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Product Information

98213-1-PBS targets CD157 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: bone marrow stromal cell antigen 1
• Calculated Molecular Weight: 35kDa
• Gene Symbol: Bst1
• Gene ID (NCBI): 12182
• RRID: AB_3745880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q64277
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD157, also known as bone marrow stromal cell antigen 1 (BST-1), or ADP-ribosyl cyclase 2, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores.