CoraLite®647 Anti-Mouse CD16/32 (93)

CD16/32 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite®647 Fluorescent Dye

CloneNo.

93

Cat no : CL647-65057

Synonyms



Tested Applications

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65057-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65057 targets CD16/32 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen VH81X Tg B cells and T220 stromal cell line
Full Name Fc receptor, IgG, low affinity IIb
GenBank accession numberBC038070
Gene symbol Fcgr2b
Gene ID (NCBI) 14130
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

Protocols

Product Specific Protocols
FC protocol for CL647 CD16/32 antibody CL647-65057Download protocol
Standard Protocols
Click here to view our Standard Protocols