CoraLite® Plus 647 Anti-Mouse CD16/32 (93)

CD16/32 Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2a, lambda

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

93

Cat no : CL647-65057

Synonyms

AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

---

Validation Data Gallery

• 

  IF Staining of mouse splenocytes using CL647-65057

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647 CD16/32 antibody (CL647-65057, Clone: 93 ) at dilution of 1:100.

• 

  FC experiment of mouse splenocytes using CL647-65057

  1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3ε (FITC-65060, Clone: 145-2C11) and 5 ul CoraLite® Plus 647 Anti-Mouse CD16/32 (CL647-65057, Clone: 93). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL647-65057

  1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3ε (FITC-65060, Clone: 145-2C11) and CoraLite® Plus 647 conjugated Rat IgG2a isotype control. Cells were not fixed.

---

Tested Applications

• Positive IF detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65057 targets CD16/32 in IF, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, lambda
• Class: Monoclonal
• Type: Antibody
• Immunogen: VH81X Tg B cells and T220 stromal cell line
• Full Name: Fc receptor, IgG, low affinity IIb
• GenBank Accession Number: BC038070
• Gene Symbol: Fcgr2b
• Gene ID (NCBI): 14130
• RRID: AB_2920247
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 665 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.